Tuesday, October 2, 2007
Clinical Chemisty
Sorry for the late update...busy with mp.
In order to prevent graft rejection, transplant recipients have to take immunosuppressive drugs for their whole life. Immunosuppressive drugs are intended to interrupt or prohibit the immunological mechanisms occurring during graft rejection by interference with the function and metabolism of activated cells.
Name of test: Cyclosporin testing
Cyclosporin is a potent immunosuppressive drug got combating tissue rejection following organ transplant. It has serious toxic side effects and is recommended to have regular drug monitoring. Cyclosporine has increased both the short- and long-term survival rates for transplant patients, especially in heart and liver operations. The rejection of grafted tissues occurs when white blood cells (lymphocytes) called T-helper cells stimulate the activity of cell-destroying (cytotoxic) T-killer cells. These T cells, along with other white blood cells like monocytes and macrophages, cause the tissue rejection of the implanted organs. Cyclosporine reduces the ability of accessory cells to produce interleukins, resulting in decreased replication of helper and killer T cells.
Testing cyclosporine levels in the blood can help ensure that drug levels are in a range that will be therapeutic for you.
Procedure of test:
Pre-treatment is needed. The drug is coated on RBCs, therefore the solubilization reagent is added to lyse the RBC and release it. Precipitation reagent is added to suspend the protein. It is then vortexed to ensure through mix and centrifuged to obtain clear supernatant. The clear supernatant is then pipetted out and place into a sample cup which is then placed into the TDX analyzer. The reagents needed are also placed inside the analyzer. TDX is used for quantitative measurement of drugs. It uses the Fluorescence Enzyme Immunoassay (FPIA) technology.
Clinical Interpretation:
If the level is too low, organ rejection may occur.
If the level is too high, it will result in toxicity. Toxic side effects produced are primarily nephrotoxicity and hepatotoxicity.
Loh Mun Jo-anne
TG02
0503324F
Sources:
http://www.rheumatology.org/public/factsheets/cyclosporine.asp?aud=pat
Clinical Diagnostics, Chapter 47: Therapeutic drug monitoring, page1155-1156
In order to prevent graft rejection, transplant recipients have to take immunosuppressive drugs for their whole life. Immunosuppressive drugs are intended to interrupt or prohibit the immunological mechanisms occurring during graft rejection by interference with the function and metabolism of activated cells.
Name of test: Cyclosporin testing
Cyclosporin is a potent immunosuppressive drug got combating tissue rejection following organ transplant. It has serious toxic side effects and is recommended to have regular drug monitoring. Cyclosporine has increased both the short- and long-term survival rates for transplant patients, especially in heart and liver operations. The rejection of grafted tissues occurs when white blood cells (lymphocytes) called T-helper cells stimulate the activity of cell-destroying (cytotoxic) T-killer cells. These T cells, along with other white blood cells like monocytes and macrophages, cause the tissue rejection of the implanted organs. Cyclosporine reduces the ability of accessory cells to produce interleukins, resulting in decreased replication of helper and killer T cells.
Testing cyclosporine levels in the blood can help ensure that drug levels are in a range that will be therapeutic for you.
Procedure of test:
Pre-treatment is needed. The drug is coated on RBCs, therefore the solubilization reagent is added to lyse the RBC and release it. Precipitation reagent is added to suspend the protein. It is then vortexed to ensure through mix and centrifuged to obtain clear supernatant. The clear supernatant is then pipetted out and place into a sample cup which is then placed into the TDX analyzer. The reagents needed are also placed inside the analyzer. TDX is used for quantitative measurement of drugs. It uses the Fluorescence Enzyme Immunoassay (FPIA) technology.
Clinical Interpretation:
If the level is too low, organ rejection may occur.
If the level is too high, it will result in toxicity. Toxic side effects produced are primarily nephrotoxicity and hepatotoxicity.
Loh Mun Jo-anne
TG02
0503324F
Sources:
http://www.rheumatology.org/public/factsheets/cyclosporine.asp?aud=pat
Clinical Diagnostics, Chapter 47: Therapeutic drug monitoring, page1155-1156
# posted by Star team @ 8:46 PM 
Comments:
<< Home
Hey,
What's the reference range for the cyclosporine test? Can the blood be sent in other anticoagulant tubes?
Martin
TG02
What's the reference range for the cyclosporine test? Can the blood be sent in other anticoagulant tubes?
Martin
TG02
hey.. juz wondering..why is there a need to measure cyclosporin samples in 0hr and 2hr (trough and peak)?
Jia Hao
Jia Hao
To Martin,
there is no specific reference range. It depends on the dasage given. usually EDTA blood is recommended. Im not too sure about other anticoagulants.
To Jiahao,
hmm, i didnt mention anything about trough and peak samples.
The trough sample is taken before cyclosporin is taken. 2 hrs later (peak), another blood sample is taken. This is for monitoring the levels of cyclosporin in the blood. It tell us how well the drug is absorbed into the bloodstream. Regular monitoring is done to ensure that it does not lead to toxicity or that the level is too low that it leads to organ rejection. It ensures that the drug is of therapeutic level.
Jo-anne
there is no specific reference range. It depends on the dasage given. usually EDTA blood is recommended. Im not too sure about other anticoagulants.
To Jiahao,
hmm, i didnt mention anything about trough and peak samples.
The trough sample is taken before cyclosporin is taken. 2 hrs later (peak), another blood sample is taken. This is for monitoring the levels of cyclosporin in the blood. It tell us how well the drug is absorbed into the bloodstream. Regular monitoring is done to ensure that it does not lead to toxicity or that the level is too low that it leads to organ rejection. It ensures that the drug is of therapeutic level.
Jo-anne
louboutin outlet
cheap rolex watches
canada goose outlet
burberry scarf
spurs jerseys
ugg boots outlet
michael kors outlet clearance
louis vuitton handbags
uggs sale
gucci outlet online
chenyingying20161202
Post a Comment
cheap rolex watches
canada goose outlet
burberry scarf
spurs jerseys
ugg boots outlet
michael kors outlet clearance
louis vuitton handbags
uggs sale
gucci outlet online
chenyingying20161202
Subscribe to Post Comments [Atom]
<< Home
Subscribe to Posts [Atom]