Tuesday, May 22, 2007

After many hours of discussing and contemplating, the star team members came to an agreement that the list of 5 diseases should be cut down to 3.

the 3 diseases we will be shortlisting are:

Murine Typhus

Sample Collection:

From the 85 year old man, we will be collecting faeces and blood samples from him.

Collection of the stool samples are for tests to determine the presence of diseases such as Salmonellosis and Campylobacteriosis.

For Salmonellosis, the faeces sample is cultured and a smear is created. Microscopic examination and gram staining is done.

For Campylobacteriosis, the faeces sample is also cultured and a smear is created. As with Salmonellosis, gram staining and microscopic examination is carried out. The only addition is dark field microscopy.

Collection of blood samples are for tests to determine the presence of the three diseases Murine Typhus, Leptospirosis and Lyme Disease.

For Murine Typhus, serum from the blood sample is tested for antibodies to the microorganism Rickettsia typhi using Immunofluorescence Assay (IFA).

For Leptospirosis, Microscopic Agglutination Test (MAT) is used, to detect Leptospira which agglutinates with commercial antibodies.

For Lyme Disease, serum is obtained to run through Enzyme Linked Immunosorbent Assay (ELISA). ELISA is done to test for IgG and IgM.

After much discussion, we have finalized the 5 possible disease the 85 year old patient is suffering from.

(1) Salmonellosis

(2) Campylobacterosis

(3) Lyme Disease

(4) Murine Typhus

(5) Leptospirosis

Wednesday, May 9, 2007

We think 2. Allegic Reaction cannot be classsify as a disease, it is just an adverse reaction to the bacteria he is exposed to. So, we've replaced it with Leptospirosis.

2. Leptospirosis is a disease cause by leptospira which is commonly found in rodents and dogs. They are capable of penetrating the skin through cuts or wounds. People are infected when exposed to water contaminated with infected animal's urine or when handling tissues of infected animals. Disease is most common in tropical months. Patients down with leptospirosis develop influenza-like illness with fever and myalgia(muscle pain) in the initial stage.

Reason: Patient's flat is infested with rats and he has a habit of feeding stray dogs and cats in which all these are the possible carriers of leptospira thus exposing himself to infection. There is also a high chance he may be exposed to the rats' urine and accidentally consumed water or food contaminated by them. Leptospirosis develop symptoms which are similar to what he is experiencing. Moreover, Singapore has a tropical climate which makes an optimum environment for Leptospirosis.

Medical Microbiology, 5th edition

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