Star Team

Sample Collection:

From the 85 year old man, we will be collecting faeces and blood samples from him.

Collection of the stool samples are for tests to determine the presence of diseases such as Salmonellosis and Campylobacteriosis.

For Salmonellosis, the faeces sample is cultured and a smear is created. Microscopic examination and gram staining is done.

For Campylobacteriosis, the faeces sample is also cultured and a smear is created. As with Salmonellosis, gram staining and microscopic examination is carried out. The only addition is dark field microscopy.

Collection of blood samples are for tests to determine the presence of the three diseases Murine Typhus, Leptospirosis and Lyme Disease.

For Murine Typhus, serum from the blood sample is tested for antibodies to the microorganism Rickettsia typhi using Immunofluorescence Assay (IFA).

For Leptospirosis, Microscopic Agglutination Test (MAT) is used, to detect Leptospira which agglutinates with commercial antibodies.

For Lyme Disease, serum is obtained to run through Enzyme Linked Immunosorbent Assay (ELISA). ELISA is done to test for IgG and IgM.