Sunday, September 9, 2007
Haematology
Cell Dyn 3500 is automated Full Blood Counter. With tons of specimens to prcess everyday, it comes along very handy as it take less than a minutes to generate results for the following:
WBC Impedance Count (WIC)
WBC Optical Count (WOC)
Neutrophils
Lymphocytes
Monocytes
Eosinophils
Basophils
RBC
Haemoglobin
Hematocrit (HCT)
MCV
MCH
MCHC
RDW
Platelets
Mean Platelet Volume (MPV) : measurement of the average size of your platelets. New platelets are larger, and an increased MPV occurs when increased numbers of platelets are being produced. MPV gives information about platelet production in your bone marrow.
WBC Optical Count (WOC) and WBC Impedance Count (WIC) are just 2 different methods used by the analyzer to assure correct leukocyte count.
The Reagents involved:
1.Diluent
Dilute samples so the analyzer can measure the various components. It also rinses aspiration probes to reduce carry-over from the previous test.
2.Lysate
-Rapidly lyse RBCs
-Strip white cells cytoplasm leaving the nucleus membrane intact so the white cells’ nuclei can be enumerated
-converts haemaglobin to a single chromogen that is measurable at 540nm
3.Detergent
4.Sheath Reagent
-osmotically lyse RBCs
-maintain light scattering properties of WOC during the measuring period
- prevent accumulation of air bubble in WOC flow system.
5.Enzymatic Cleaner
-removes protein build-up within the analyzer
Blood can be collected via venipuncture or finger-prick and sent to the lab in EDTA tubes (big purple tube and small pink tubes). After the blood has been checked for clots and is well-mixed, it is loaded into the analyzer. Clotted blood tubes are rejected as they cause low platelet count.
CD 3500 is able to aspirate blood in 2 modes- OPEN and CLOSE. Open mode involves manual positioning of blood tube to the aspirating probe. In the case of Close mode, the blood tubes just need to be place in the cartridge and the probe will automatically mix and aspirate blood.
After blood is aspirated into the Shear Valve (inside the analyzer), the valve will cut the blood into different segment for various testing.
WBC
1. WIC
Blood will be diluted and mixed with lysis solution. The diluted sample passes through a thin aperture where electrical impedance is used to count the WBC as they transverse through the aperture.
2. WOC
Blood is diluted and transferred to the WOC flow cell by the peristaltic pump. A laser beam is focused on the flow cell, as the sample stream (one cell at a time) intersects the laser beam, the light scattered by the cells is measured.
RBCs and Platelets
Blood is diluted 10,812 times. Electrical impedance is used to count RBCs and platelets as they transverse through the aperture.
Haemaglobin
Hb concentration is measured spectrophotometrically. Lysis solution converts Hb to hemiglobinhydrozylamine complex. Light-emitting didode (LED) with a wavelength of 540nm determines the amount of Hb, by capturing the light emitted, by a photodetector.
The rest of the parameters are reported based on the calculation automatically done by the analyzer.
cheers,
phuiyuen
WBC Impedance Count (WIC)
WBC Optical Count (WOC)
Neutrophils
Lymphocytes
Monocytes
Eosinophils
Basophils
RBC
Haemoglobin
Hematocrit (HCT)
MCV
MCH
MCHC
RDW
Platelets
Mean Platelet Volume (MPV) : measurement of the average size of your platelets. New platelets are larger, and an increased MPV occurs when increased numbers of platelets are being produced. MPV gives information about platelet production in your bone marrow.
WBC Optical Count (WOC) and WBC Impedance Count (WIC) are just 2 different methods used by the analyzer to assure correct leukocyte count.
The Reagents involved:
1.Diluent
Dilute samples so the analyzer can measure the various components. It also rinses aspiration probes to reduce carry-over from the previous test.
2.Lysate
-Rapidly lyse RBCs
-Strip white cells cytoplasm leaving the nucleus membrane intact so the white cells’ nuclei can be enumerated
-converts haemaglobin to a single chromogen that is measurable at 540nm
3.Detergent
4.Sheath Reagent
-osmotically lyse RBCs
-maintain light scattering properties of WOC during the measuring period
- prevent accumulation of air bubble in WOC flow system.
5.Enzymatic Cleaner
-removes protein build-up within the analyzer
Blood can be collected via venipuncture or finger-prick and sent to the lab in EDTA tubes (big purple tube and small pink tubes). After the blood has been checked for clots and is well-mixed, it is loaded into the analyzer. Clotted blood tubes are rejected as they cause low platelet count.
CD 3500 is able to aspirate blood in 2 modes- OPEN and CLOSE. Open mode involves manual positioning of blood tube to the aspirating probe. In the case of Close mode, the blood tubes just need to be place in the cartridge and the probe will automatically mix and aspirate blood.
After blood is aspirated into the Shear Valve (inside the analyzer), the valve will cut the blood into different segment for various testing.
WBC
1. WIC
Blood will be diluted and mixed with lysis solution. The diluted sample passes through a thin aperture where electrical impedance is used to count the WBC as they transverse through the aperture.
2. WOC
Blood is diluted and transferred to the WOC flow cell by the peristaltic pump. A laser beam is focused on the flow cell, as the sample stream (one cell at a time) intersects the laser beam, the light scattered by the cells is measured.
RBCs and Platelets
Blood is diluted 10,812 times. Electrical impedance is used to count RBCs and platelets as they transverse through the aperture.
Haemaglobin
Hb concentration is measured spectrophotometrically. Lysis solution converts Hb to hemiglobinhydrozylamine complex. Light-emitting didode (LED) with a wavelength of 540nm determines the amount of Hb, by capturing the light emitted, by a photodetector.
The rest of the parameters are reported based on the calculation automatically done by the analyzer.
cheers,
phuiyuen
# posted by Star team @ 8:23 PM 
Comments:
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hey phui yuen,
You mentioned that there are two modes to aspirate blood, how do decide which mode to use?
meet up soon, yea? =)
Jo-anne Loh
TG02
You mentioned that there are two modes to aspirate blood, how do decide which mode to use?
meet up soon, yea? =)
Jo-anne Loh
TG02
Hey phui yuen
When do u decide which method (WOC or WIC)to use to assure the correct leukcoyte count?, is it based on the clinical diseases? If so can u give some egs which uses WOC to determine the leukocyte count. (and also for WIC) How do both of them work? see ya! =)
Eugene Wong
TG02
When do u decide which method (WOC or WIC)to use to assure the correct leukcoyte count?, is it based on the clinical diseases? If so can u give some egs which uses WOC to determine the leukocyte count. (and also for WIC) How do both of them work? see ya! =)
Eugene Wong
TG02
Joan-NE :)
the 2 mode - open and close. For open mode, cos its manually holding the tube for the probe to aspirate, both adult(big purple) and paediatric(small pink) EDTA tube can be used.
For closed mode, only the adult EDTA tube can fit into the cartridge/holder.
So, we'll only select close mode when there's a lot of adult EDTA tube coming in which is ard after lunch time. yep :)
Eugene:
WOC and WIC cannot be manually selected, its a method auto-selected by the analyser. For every sample, the analyser will used WIC and WOC to determine the leukocyte count (it's not based on any disease). WIC and WOC are just 2 different methods to determine leukocyte count. I hope i answered your question ;)
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the 2 mode - open and close. For open mode, cos its manually holding the tube for the probe to aspirate, both adult(big purple) and paediatric(small pink) EDTA tube can be used.
For closed mode, only the adult EDTA tube can fit into the cartridge/holder.
So, we'll only select close mode when there's a lot of adult EDTA tube coming in which is ard after lunch time. yep :)
Eugene:
WOC and WIC cannot be manually selected, its a method auto-selected by the analyser. For every sample, the analyser will used WIC and WOC to determine the leukocyte count (it's not based on any disease). WIC and WOC are just 2 different methods to determine leukocyte count. I hope i answered your question ;)
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