Star Team

1. Acces to the lab is limited when experiments with culture are in progress
2. Eating, drinking, chewing gum or smoking are not permited in the lab
3. Mouth pipetting is prohibited
4. All procedures are performed carefully to minimize the creation of splashes &/or aerosol
5. In the BSL1, there are sinks for handwashing. All personnel must wash their hands, after they handle viable materials, after ther remove their gloves, before they leave the lab
6. Work surfaces are decontaminated at least once a day with 1:50 dilution of 5.25% NaOCl
7. If any spillage of micro-organism or chemicals occurs, the spillage area must be soak with 1:5 dilution of 5.25% NaCl
8. All cultures, stocks, contaminated needles, sharp equipments, broken glasses(such as glass pipettes), must be decontaminated before disposal, by autoclaving
9. Broken glasses must not be handled directly by hand but must be remove by a brush and dustpan
10. A biohazrad sign must be posted at the entrance of the lab. This only occurs if infectious agents are persent. The sign must include the name of agents i use, the name and phone no. of the investigator
11. Personnel Protective Equipments (PPE) such as gloves, lab coat must be worn in the lab at all times
12. Goggles should be worn, if the personnel wears contact lense or does not wear spectacles, when splashes of hazrads chemical or micro-organism is anticipated
13. Bench top in BSL1 are impervision to water and are resistant to organic solvent, acids, alkalis, moderate heat.
14. The bench top n BSL1 is also resistant to chemicals which are used to decontaminate the work surface and equipments
15. BSL 1 is a "pressured" laboratory. That is it prevents dirty air from entering the lab when the door is open

Biosafety Laboratory 2

Biosafety Laboratory 2 is similiar to BSL 1 lab. it is suitable for work involving agents of modertate potential hazard to the personnel and the environmnet. Nevertheless there are some differences. In BSL2, lab personnel have specific training in handing pathogenic agents and are directed by a trained personnel. Acces to the lab is restricted when work is being conducted. Extreme precautions are taken with contaminated sharp items and certain procedure in which infectious aerosol or splahes may be created in the biosafety cabinets.9details are below)
The standards, special practices, safety equipments and facilities apply to agents assigned to BSL1(point 1-15) also applies to BSL2. In AS4 , instead of BSL2,AS4 has 2 BSL 2 plus lab. One for micro-organism work while another one is for mammalian cell culture work

Apart from those ponits, there are a few more points personnel must follow

1. The laboratory director establishes polices and procedure whereby people who have been advised of potential hazards and meet specific entry requirements may enter the laboratory
2. Biosafety procedures are incorporated in to Standard Operating Procedures (SOPs) for the lab by the lab director
3. Personnel working in BSL2 are advised of specific hazards anf are required to read and follw instructions on practices and procedures
4. All personnel working in BSL2 must recieve appropriate training on the potential hazards associated with the work involved, the necessary precautions to prevent exposure to hazardous materials and evacuation procedures
5. Personnel Protective Equipments designated for BSL2 use are worn in BSL2. This protective clothing is removed and left in the lab before leaving for other lab or non-lab areas

I hope you guys had fun reading and understood everything. I hope I am able to answer all your questions. Good Luck and Good bye for now.

Eugene Wong TG02

# posted by Star team @ 9:46 AM