Saturday, June 30, 2007
Medical Microbiology
Name of Test:
Light Diagnostic Cytomegalovirus (CMV) pp65 protein in periheral blood leukocytes
Principle of Test:
The test utilises the indirect immunofluorescence technique for identifying human CMV pp65 antigen in the blood leukocytes. Monoclonal antibody is added and bound to CMV pp65 antigen on formalin fixed leukocytes and incubated. Excess antibody is washed with phosphate buffered saline (PBS). Fluorescein isothiocyanate (FITC) conjugated antibody, also known as secondary antibody, is bound to the antigen-antibody complex and incubated. Excess antibody is washed with PBS. FITC exhibits green fluorescence when excited by ultraviolet light allowing visualization of antigen-antibody complex by fluorescence microscopy.
Test Result with reference range:
Control slides should be prepared from known positive and negative blood samples, for each batch of test. Positive result emits green light, while negative result emits dull red.
Clinical interpretation:
The test is used for the detection of CMV in blood leukocytes, which is closely associated with the clinical manifestation of the CMV disease. This will be useful in the diagnosis of CMV infection. The test is rapid and sensitive for detecting CMV in isolated leukocytes; this will prevent any delay in treatment. However, if negative and positive controls cannot be clearly distinguished, the test is considered invalid.
Student name: Foo Yong Yang
Light Diagnostic Cytomegalovirus (CMV) pp65 protein in periheral blood leukocytes
Principle of Test:
The test utilises the indirect immunofluorescence technique for identifying human CMV pp65 antigen in the blood leukocytes. Monoclonal antibody is added and bound to CMV pp65 antigen on formalin fixed leukocytes and incubated. Excess antibody is washed with phosphate buffered saline (PBS). Fluorescein isothiocyanate (FITC) conjugated antibody, also known as secondary antibody, is bound to the antigen-antibody complex and incubated. Excess antibody is washed with PBS. FITC exhibits green fluorescence when excited by ultraviolet light allowing visualization of antigen-antibody complex by fluorescence microscopy.
Test Result with reference range:
Control slides should be prepared from known positive and negative blood samples, for each batch of test. Positive result emits green light, while negative result emits dull red.
Clinical interpretation:
The test is used for the detection of CMV in blood leukocytes, which is closely associated with the clinical manifestation of the CMV disease. This will be useful in the diagnosis of CMV infection. The test is rapid and sensitive for detecting CMV in isolated leukocytes; this will prevent any delay in treatment. However, if negative and positive controls cannot be clearly distinguished, the test is considered invalid.
Student name: Foo Yong Yang
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